Real-Time PCR analysis of microbial composition in white spot lesions associated with fixed orthodontic appliances

Document Type : Original Article

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Department of Microbiology, College of Dentistry, University of Babylon, Babylon, Iraq

Abstract

Background: Orthodontics is a specialty of dentistry that deals with the diagnosis, prevention, and correction of malpositioned teeth and jaws. It is designed to correct oral health issues and incorrect jaw position, as well as improve the appearance of the teeth and face. Fixed orthodontic appliances are non-removable; hence, the presence of nooks and crannies encourages the accumulation of food and plaque, which makes oral hygiene challenging and even leads to dental pathologies. Objective: The study’s objective  is to molecularly  analyze of micro-organisms in white spot lesions caused by fixed orthodontic appliances. Methods: A total of 100 individuals, including 50(50%) patients and 50 (50%) controls, 36 males and 64 females, aged 15-35 years, underwent fixed orthodontic treatment for those measuring the microbial load in dental plaque around the bracket for at least one month after activation. Results: The findings showed that the case group had higher significant difference as opposed to the control group with  p value = 0.002 in the count and  p value = 0.003 in presence of Streptococcus mutans, which determined by real-time PCR .Conclusion: In contrast to the control group, which is free of WSLs, case group had a higher load and existence of Streptococcus mutans.

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