Preparation of a new culture medium by using Gonaderma lucidum for cultivation of Leishmania donovani promastigote parasite

Mohammed, Sabaa Taher; Ghazi, Hanan Faisal; Ahmed, Haider Yousif; Majeed, Hadeel Abdulatif; Ajah, Hamzia Ali; Ali, Ekhlass N.; Ali, Luma Qasim

doi:10.21608/mid.2025.377492.2724

Preparation of a new culture medium by using Gonaderma lucidum for cultivation of Leishmania donovani promastigote parasite

Document Type : Original Article

Authors

¹ Department of Biology, Collage of Science, Al –Mustansiriyah University, Baghdad, Iraq

² Department of Microbiology, Collage of Science, Al –Mustansiriyah University, Baghdad, Iraq

10.21608/mid.2025.377492.2724

Abstract

Background: Leishmaniasis is a parasitic disease caused by the bite of infected sandflies carrying the Leishmania parasite. It's classified as a neglected tropical disease and affects many regions in the world, particularly in developing areas. Aim: Ganoderma lucidum powder is used to prepare a new culture medium for growing the Leishmania parasite in vitro, which consists of two stages. Methods: Ganoderma lucidum powder was used to prepare the solid phase instead of brain heart. This medium was attended by two groups. The first group prepared the solid phase of the powder of the Ganoderma, the second group prepared only the liquid phase was the filtrate of the Ganoderma. The third group was NNN media as a control group. Results: The parasite growth continued in a good way for a period of time. More than 20 days, with good vitality and natural shape, and without making a secondary subculture, increased numbers of parasites in the new Ganoderma powder medium, which was manufactured by adding Ganoderma powder to the solid phase, was a better increase than the medium in which Ganoderma powder was used in the manufacture of the liquid phase. Only its highest rate was on the eighth day, as it reached (54.5 and 48.7) x 10⁴ cells/ml, respectively, compared with NNN-media, which amounted to (58.4 x 10⁴ cells/ml), then the parasite preparation started decreasing with time until it became 20 days 0, 3 x 10⁴ cells/ml, respectively, while the NNN-media medium reached (6.5 x 10⁴) cells/ml, with significant differences between the two media. Conclusion: In both groups media, the results indicated an increase in the number of parasites counted over twenty days, with the highest increase occurring on the eighth day.

Keywords

Main Subjects