Effect of different carbon and nitrogen sources on biofilm formation of Acinetobacter baumannii

Document Type : Original Article

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College of Health and Medical Techniques, Middle Technical University (MTU), Baghdad, Iraq

Abstract

Background: Biofilm is the major problem in patients suffering from antibiotics resistance because it protects the bacteria from many environment effectors. The hospital infections are one of the most important pathogens which increase by A.baumannii Aim: Detection of biofilm and factors affecting on biofilm formation in A. baumannii. Methods: One -hundred of A. baumannii isolates were collected from patients suffering from different complaints such as surgical wound infections, burns infection, urinary tract infection and pulmonary infections. Biofilm formation detection and the nitrogen and carbon sources affecting on biofilm production done by microliter plate method. Results: The results of the current study observed there were (34%) of A. baumannii bacterial isolates produced strong biofilm out of 100 cases, high production of biofilm was with Trypton and yeast extract sources than the other nitrogen sources, while; the carbon sources including fructose, sucrose and lactose were the good sources for biofilm formation for the 9 isolates. Conclusion: The highest percentage of isolation (42%) was of sputum and the lowest percentage (3%) was from urine. A. baumannii produced biofilm with three scores (34%) strong biofilm, (13%) moderate biofilm, and (53%) weak biofilm, quantitatively the carbon sources were more good than nitrogen for biofilm formation.

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