Effective antifungal compounds of Lactobacillus casei against vulvovaginal candidiasis for women in the Thi-Qar Province

Mohammed, Haneen A.; Alfayyadh, Iman

doi:10.21608/mid.2025.355448.2483

Effective antifungal compounds of Lactobacillus casei against vulvovaginal candidiasis for women in the Thi-Qar Province

Articles in Press

Document Type : Original Article

Authors

Haneen A. Mohammed ¹
Iman alfayyadh ²

¹ Pathological Analysis Department, College of Science, University of Thi-Qar, Thi-Qar, Iraq

² 2Department of Pathological Analysis, College of Science, University of Thi-Qar, Thi-Qar, Iraq

10.21608/mid.2025.355448.2483

Abstract

Background: Vulva and vagina is the hallmark of vulvovaginal Candidiasis (VVC), a fungal illness usually brought on by the yeast Candida albicans, while some species of Candida are normally found in the genital tract under some situations they can multiply excessively and cause symptoms including vaginal itching, pain and discharge. Methods: From 48 patients infected with vulvovaginal candidiasis, L.casei (ATCC39392) extracted and chemical compounds identified by Gas Chromatography, the extract of L. casei inhibited C. albicans in well diffusion assay and the inhibition zone, its was 15 mm, while minimum inhibiter concentration was 32 μg/ml, examine effected of extract as biofilm. Results: The results showed that 20.8% of isolates formed strong biofilm while moderate and weak 70.8% and 8.3% respectively. The extracted had significant effect as antibiofilm, it was able to reduce biofilm strongly 60%, moderate 20% while there was 20% not effected. Examination cytotoxicity of this extracted showed that there is low toxicity on normal cells of dermal cell line and hepato cell line, Ic50 was and 192.7µs/ml for both WRL-68 and HdFn cell lines, which inhibition rate 14.2 and 16.1 for HdFn and WRL-68 respectively at concentration 400 µg /ml. Conclusion: Lactic acid bacteria produce compounds that exterminate fungi. These chemicals effectively inhibit the formation of Candida biofilms. The extract of Lactobacillus casei exhibits minimal cytotoxicity on normal dermal and hepatic cell lines.

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