Incidence of resistance pattern and biofilm-forming genes of Acinetobacter baumannii isolated from Iraqi children

Document Type : Original Article

Authors

1 Microbiology Department, College of Science, Mustansiriyah University, Baghdad, Iraq

2 Department of Medical Laboratory Techniques, Technical Institute of Balad, Middle Technical University, Baghdad, Iraq

3 Imamein Kadhimein Medical city, Baghdad, Iraq.

4 Central Teaching Hospital of Pediatrics in Baghdad, Iraq

Abstract

Background: Acinetobacter baumannii is frequently implicated in nosocomial infections, causing a diverse range of illnesses in humankind. The objective of the study was to investigate the multidrug-resistant and biofilm-forming A. baumannii isolated from Iraqi children. Methods: Seventy-five blood and urine samples were collected from children in Baghdad, Iraq. VITEK 2 compact was used for identification and antibiotic susceptibility testing. Biofilm production was assessed using microtiter plates, and biofilm genes such as csuE, pgaB, bfmS, and ompA were detected using conventional PCR. Results: Of the sample size, 54 isolates were identified as A. baumannii. Among the tested antibiotics, ticarcillin (68.5%) showed the highest resistance rate, while colistin showed the highest susceptibility rate (92.6%). Furthermore, 61.11% of the isolates were XDR, and 29.6% were MDR. All the isolates showed the capability for biofilm production; 63% and 37% of isolates produced robust and moderate biofilm, respectively. All the isolates were determined to contain a minimum of one gene relevant to biofilm formation; however, the most prevalent gene was csuE (100%). Conclusion: High resistance rates were shown among A. baumannii strains. Moreover, biofilm gene csuE was shown in all the strains. However, further experimentation to evaluate biofilms' involvement in the pathophysiology of A. baumannii infections is mandatory.

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