Document Type : Original Article
Authors
1
Clinical Pathology Department, National Liver Institute, Menoufia University, Menoufia, Egypt
2
Department of Hepatology and Gastroenterology, National Liver Institute, Menoufia University, Menoufia, Egypt
3
Department of Clinical and Molecular Parasitology, National Liver Institute, Menoufia University, Menoufia, Egypt
4
Department of Epidemiology and Preventive Medicine, National Liver Institute, Menoufia University, Shebin El Kom, Menoufia, Egypt
5
Department of Pathology, National Liver Institute, Menoufia University, Shebin El Kom, Menoufia, Egypt.
6
Department of Clinical and Chemical Pathology, Faculty of Medicine, Zagazig University.
Abstract
Background: Helicobacter pylori (H. pylori) is considered a significant cause of gastric diseases. It was linked to gastric carcinoma and lymphoma. Aim: The present study aimed to investigate the association between the genetic polymorphism of the ABCB1 gene (C1236T/C3435T) and the susceptibility to H. pylori infection and the response to therapy. Methods: 150 patients were enrolled. One hundred patients with positive H. pylori infection, and 50 negative control. Real-time Polymerase Chain Reaction (PCR) was used to detect C1236T (rs1128503) and C3435T (rs1045642) single nucleotide polymorphisms (SNPs). Results: The susceptibility of H. pylori infection was influenced by C1236T SNPs. The CC and CT genotypes showed a significant risky association with infection (OR = 8.3, 95%CI: 1.8 – 47.3) and (3.6, 95%CI: 1.11 – 12.4), respectively; p-value= 0.009). C allele was significantly associated with an increased risk of H. pylori infection (OR = 2.8, 95% CI: 1.4 – 5.9; p-value= 0.001). A significant association was found between the failure of treatment and the SNP C1236T (p < 0.01), especially the CT and CC genotypes (OR = 3.1, 95% CI: 1.3 – 7.1) and (5.1, 95%, CI: 1.4 – 18.2, respectively). Also, the C allele was significantly associated with an increased risk of treatment resistance (OR= 2.08, 95% CI: 1.07 – 3.7; p-value= 0.01). Conclusion: The frequencies of the C1236T [rs1128503] genotypes between cases and control were significantly different and was identified as a significant factor influencing the risk of H. pylori infection in addition to the outcomes of eradication regimen in Egyptian populations.
Keywords
)
View on SCiNiTO