Microbiological quality and antibiotic resistance profile of enteropathogenic Escherichia coli isolated from farm milk, curdled milk and fresh milk sold of Ouagadougou and Pabre, Burkina Faso

Waongo, Blaise; CISSE, Hama; SOMDA, Namwin Siourimè; Zio, Souleymane; Bougma, Sanogo; Kaboré, Boukaré; Tankoano, Abel; SAVADOGO, Aly

doi:10.21608/mid.2024.297942.2009

Microbiological quality and antibiotic resistance profile of enteropathogenic Escherichia coli isolated from farm milk, curdled milk and fresh milk sold of Ouagadougou and Pabre, Burkina Faso

Document Type : Original Article

Authors

¹ Département de Biochimie-Microbiologie, Laboratoire de Biochimie et d’Immunologie Appliquées, Université Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso

² Département Technologie Alimentaire (DTA), Centre National de Recherche Scientifique et Technologique (CNRST) / Institut de Recherche en Sciences Appliquées et Technologies (IRSAT) / Direction Régional de L’Ouest, 03 B.P.2393 Bobo - Dioulasso 03, Burkina Faso

³ Laboratory of Applied Biochemistry and Immunology, Department of Biochemistry and Microbiology, University Joseph KI-ZERBO, 03 BP 7021 Ouagadougou 03, Burkina Faso

10.21608/mid.2024.297942.2009

Abstract

Milk is a highly perishable foodstuff, which can be contaminated by pathogenic microorganisms. Among these pathogens, enteropathogenic Escherichia coli (EPEC) are known to be responsible for diarrhea in children aged 0-5 years. Aim of this study is to assess microbial load of milk, characterize EPEC strains in curdled and farms milk from markets of Ouagadougou and Pabre cities then assess their antibiotics resistance profile. Standard methods were used for pH and microbiological quality assessment. EPEC were identified by serotyping, and their antibiotic resistance profile was assessed according to CASFM. A total of 102 milk samples were collected. Milk pH values ranged from 3.35 to 6.82, with averages of 6.61 for fresh milk, 3.77 for curdled market milk and 6.54 for farms milk. Values of total aerobic mesophilic flora (FAMT) ranged from (1.70±1.10) ×107 to (3.56±1.37) ×108 CFU/mL, with an average of (3.76±1.63) ×108 CFU/mL. Total coliforms (TC), values ranged from (1.94±0.84) ×105 CFU/mL to (2.00±1.00) ×108 CFU/mL, with an overall average of (3.40±1.72) ×107 CFU/mL. Thermotolerant coliform (TCh) averages for fresh milk sampled at markets ranged from (1.47±0.52) ×103 CFU/mL to (2.50±1.01) ×105 CFU/mL. A total of 125 E. coli strains were isolated, of which 9 (7.2%) were enteropathogenic. Study’s showed total resistance to penicillin, cefazolin, streptomycin, fusidic acid and tetracycline. However, they were sensitive to cotrimoxazole (100%), chloramphenicol (100%), nitrofurantoin (88.89%), gentamycin (66%) and fosfomycin (55.55%), respectively. Study’s was revealed that curdled and fresh milks sold in Ouagadougou contained pathogenic, some of which were resistant to commonly used antibiotics.

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