Study the relationship between antibiotic resistance and the biofilm-forming ability of bacteria isolated from cases of osteomyelitis after surgery

Document Type : Original Article

Authors

1 Biology Department College of Science / University of Mosul, Mosul City, Iraq

2 Microbiology Department, Ninevah College of Medicine, Ninevah University, Mosul City, Iraq

Abstract

Background: Biofilms play an important role in protecting bacteria from antibiotics, which makes it difficult to treat and heal, in addition to the occurrence of bacterial resistance to antibiotics due to the long treatment period, in the case of bone inflammation after surgical operations. Objectives: One of the most important objectives of this study is to investigate the correlation between antibiotic resistance and the ability to form biofilms of bacteria isolated from cases of Osteomyelitis.  Methods: Sixty Osteomyelitis samples have been collected, The Ages of patients included in this study are between (7- 70) years old, among whom 11(18.34%) were Females and 49(81.66%) were males. Three types of culture media were used for primary isolation (nutrient agar, MacConkey agar, and blood agar). Results: The results of the initial isolation showed that 56 samples out of 60 gave positive results for the bacterial infection, with 67 bacterial isolates. The ability of the isolates to form biofilms was evaluated in several ways, the first method on medium Congo Red Agar, the second by Microtiter Plate Method, and the third by Scanning Electron Microscope technology. The percentages were for Congo Red Agar (62.5% strong, 25% moderate, and 12.5% mild) and by the Microtiter Plate Method (50% strong, 25% moderate, 25% mild). By Scanning Electron Microscope technology, biofilm formation steps were proven by examining the biofilm of Escherichia coli. The aggregation of bacteria forming biofilms was confirmed by staining the bacterial swabs. Biofilm-specific gene (icaC) and (FimH) for S. aureus and E. coli respectively. Azithromycin resistance gene sequences (msrA) for S. aureus and (mphA) for E. coli. The results showed that there was a 100% correlation between the two types of genes in both types of bacteria. Conclusion: Osteomyelitis is considered a multifaceted infection, because the bacterial species that make up the biofilms are diverse, which leads to their resistance to a large number of antibiotics.

Keywords

Main Subjects

Microbes and Infectious Diseases

Articles in Press, Accepted Manuscript
Available Online from 14 August 2024

Files

Share

How to cite

Statistics