Application of selenium Nanoparticles biosynthesized by Serratia marcescens compared with commercial selenium Nanoparticles

Selah, Mohammad T.; Mohammad, Ghada A.

doi:10.21608/mid.2024.302770.2060

Application of selenium Nanoparticles biosynthesized by Serratia marcescens compared with commercial selenium Nanoparticles

Document Type : Original Article

Authors

¹ Nineveh Education Directorate, Mosul, Iraq

² Department of Biology, College of Science, University of Mosul, Mosul, Iraq

10.21608/mid.2024.302770.2060

Abstract

Background: The biosynthesis of nanoparticles by bacteria is considered safe, not harmful to the environment, and less cost than the chemical and physical methods. The study aimed to use Serratia marcescens for biosynthesizing selenium nanoparticles (SeNPs). Methods: To prove that, SeNPs biosynthesized were characterized by seven techniques. UV-visible spectroscopy exhibited maximum peak at 274 nm, FT-IR analysis showed the appearance of new functional group, The zeta potential analysis revealed that the SeNPs had acharge of -30.06 mv, the Atomic Force Microscopy demonstrated the size of SeNPs as an average size 70.77 nm, while the Field Emission Scanning Electron Microcopy gave range for size of SeNPs between 33.49 to 167 nm. Energy Dispersive X-ray technique revealed that SeNPs exist percentage reached to 43.4%, X-Ray Diffractometer showed the SeNPs had crystalline. Results: A standard microplate was used to investigate the biological activity of biosynthesized SeNPs and compared it with commercial SeNPs to determine their inhibitory effect against 4 pathogenic slandered bacteria: Streptococcus pyogenes, Listeria monocytogenes, Salmonella enterica and Acintobacter baumannii. The results showed that biosynthesized SeNPs was superior in inhibitory activity than commercial SeNPs. Antioxidant activity was tested. The biosynthesized SeNPs proved the effectiveness as an antioxidant more than commercial SeNPs. Conclusion: Our findings concluded that this is the first time that Serratia marcescens has been shown to be succeeded to synthesis SeNPs. And Strong antibacterial and antioxidant activity of these SeNPs suggests apotential future for their use in avariety of applications.

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