Evaluation of inhibitory action of bacteriophage against Multi-drug resistant Escherichia coli

Budhathoki, Sumi; Kandel, Susma; Chand, Dibbya; Kunwar, Saroj; Poudel, Govinda Akela; Shrestha, Ram Krishna; Shah, Ashok Kumar; Jaiswal, Suresh; Thakur, Rajesh Kumar; Nepal, Rupa

doi:10.21608/mid.2024.297320.1995

Evaluation of inhibitory action of bacteriophage against Multi-drug resistant Escherichia coli

Document Type : Original Article

Authors

¹ Department of Laboratory Medicine, Modern Technical College, Pokhara University, Lalitpur, Nepal

² School of Medical and Allied Science, Galgotias University, Uttar Pradesh, India

³ School of Health and Allied Sciences, Pokhara University, Pokhara, Nepal

10.21608/mid.2024.297320.1995

Abstract

Background: Bacteriophages are ubiquitous in the environment where their host resides such as rivers, soil, sewage, poultry, or animal feces, and seawater. thus, the present study aims to isolate the multidrug-resistant (MDR) Escherichia coli (E. coli) from various clinical specimens of patients and to inhibit these MDR E. coli by bacteriophage. Methodology: A Hospital-based cross-sectional study was carried out in the Microbiology laboratory of Star Hospital, Sanepa, Lalitpur from June- August 2021. 175 Clinical isolates of E. coli positive culture of E. coli and bacteriophage against E. Coli. were taken for Isolation of bacteriophage (plaque assay) using standard microbiological methods and Isolation of bacterial strains followed by purification of phages and determination of host spot test. Ethical consideration was taken from the IRC of Star Hospital Research Council. The data was entered in Ms. Excel 2013 and analyzed by SPSS version 17. Results: Out of 3 sewages, 2 phages were isolated named as $E against Escherichia coli. Phages showed positive results against Escherichia coli and $E phage was found to be large, and clear from plaque assay. Total 70 E. coli was tested against 10 antibiotics out of which 37(52.9%) were MDR E. coli and 33(47.1%) were non MDR E. coli. Among 70 samples of E. coli, 38(54.3%) showed to be sensitive and 32(45.7%) showed to be resistant to isolated bacteriophage. Among 37 MDR E. coli isolates, 17(44.7%) were found to be sensitive and 33 Non-MDR E. coli, 21(55.3%) were found to be sensitive. Conclusion: Bacteriophages can be used to treat the infection caused by E. coli which are MDR.

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