Phylogenetic analysis of Staphylococcus sp. related with thyroiditis in Al-Nasiriyah city, Iraq

Document Type : Original Article

Authors

1 Laboratory Specialist, Ministry of Health, Thi-Qar Health Directorate, Al-Haboby Teaching Hospital, Thi-Qar, Iraq

2 Department of Pathological Analysis, College of Science, University of Thi-Qar, Thi-Qar, Iraq

Abstract

Background: Staphylococcus is a Gram-positive (GM+) facultative anaerobic, non-motile cocci. It's frequently results in a variety of illnesses in human. Staphylococcus sp. considered the most common organisms that can infect the thyroid gland. Methods: Genomic DNA was extracted from 20 thyroiditis patients' blood samples and analyzed for 16S rRNA using PCR, employing the Universal gene. Gene amplification was performed in a thermocycler under specific cycling conditions, including initial denaturation, annealing, extension, and final extension. For the purpose of partially DNA sequencing for the 16S rRNA gene, six PCR results were selected in order to investigate any possible association between the results and the global records in GenBank. The data was analyzed using Statistical Package for Social Sciences (SPSS) version 27, using chi-square test and basic ratios, with P-values between 0.05 and 0.01 indicating statistical significance. Results: Findings showed that 6/20 (30%) were Staphylococcus sp. isolates. The 16S rRNA PCR product was registered in GenBank in accordance with the official accession numbers of (PP396084, PP396083, PP396085, PP396086, PP396082 and PP396093). The local Staphylococcus sp. isolates have distinct molecular links with those of similar strains worldwide according to the phylogenetic tree generated by the MEGA-10 program. Conclusions: Thyroiditis continue to be one the most threating health problems around the world. Thyroiditis patients are more susceptible to GM+ microorganisms. Staphylococcus sp. is an important opportunistic pathogen and it's associated with substantial morbidity rate.

Keywords

Main Subjects

Microbes and Infectious Diseases

Articles in Press, Accepted Manuscript
Available Online from 17 July 2024

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