Detection of Helicobacter pylori infection by three invasive methods in gastric disorders patients from Mosul City /Iraq

Document Type : Original Article

Authors

Department of Biology, College of Science, University of Mosul, Mosul, Iraq

Abstract

Background: The identification of H. pylori in Iraq lacks a standardized technique and there is limited information on the efficacy of different diagnostic methods. Objectives: The research aims to determine the prevalence of H. pylori and evaluate the effectiveness of invasive techniques. Method: Gastric biopsy specimens were collected from 50 patients with gastric disorders to detect H. pylori infection by Rapid Urease Test (RUT), histopathological examination, and ure A gene detection by PCR. Patients were classified into H. pylori -positive cases and negative cases by testing positive with at least two of the three testing methods, according to the case definition used in the study. Result: Out of the 50 cases, 58% were H. pylori -positive cases, 26% were H. pylori -negative cases, and the last 16% were indeterminate cases according to cases definition. Females and older age individuals had the highest prevalence of H. pylori infection. The ure A gene detection by PCR was the most sensitive method, followed by histopathological examination and RUT testing, with sensitivities of 96.6%, 86.2% and 79.3% respectively. Histopathology examination was the most specific with a specificity of 95.2%, followed by ure A gene PCR with 85.7%, while RUT was the least specific with a specificity of 81%. Conclusion: The current study revealed a moderate prevalence of H. pylori infection compared with previous studies. the sensitivity and specificity of the three invasive methods varied, and a combination of two methods may be necessary for a definitive diagnosis of   H. pylori infection.

Keywords

Main Subjects

Microbes and Infectious Diseases

Articles in Press, Accepted Manuscript
Available Online from 12 July 2024

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