Antibacterial activity of aluminum potassium sulfate and Lepidium sativum seeds against Vibrio cholera compared to doxycycline and tetracycline

Document Type : Original Article


1 Department of Microbiology, Al-Shomali General Hospital, Babylon Health Directorate, Babylon, Iraq

2 Department of Medical Laboratory Technology, College of Medical Technology, The Islamic University, Najaf, Iraq


Background: Cholera is a severe lethal secretory diarrheal disease caused by the Vibrio cholerae with a serious threat to public health globally particularly in developing countries. Aluminum potassium sulfate (Alum) it is crystallized double sulfate, generally odorless, colorless and crystalline solids, which is used as an antiseptic. Lepidium sativum is an erect, branched, glabrous herb with 60 cm height. The aim of the study to evaluate activity of Alum and Lepidium sativumseeds extract against clinically isolated Vibrio cholera. Material and methods: V. cholera o1 serotype ogawa were isolated from fifteen Iraqi patients. Isolates were obtained from watery diarrheal stool then cultured in peptone water for 8 hrs followed by overnight culturing on blood agar, MacConkey agar and TCBS agar, then they were diagnosed following the standard diagnostic methods. Isolates confirmed by VITIK-2.  Alum concentration was 2%, by dissolving 2grams of white alum in 100 ml distilled water then putting the solution in autoclave 120 ℃ fore 20 min. The antibiotic susceptibility testing was done by the standard well diffusion method on Mueller-Hinton agar. Results: The antibacterial activity of 2% alum was 100 % active against V. cholera, while the activity of doxycycline and Tetracycline were 86% and 80% respectively. Lepidium sativum seeds showed no activity against isolates. Conclusions: Alum is promising agent for V. cholera treatment; it is superior to both doxycycline and Tetracycline. Lepidium sativum seeds revealed no activity against V. cholera.


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