Linking 5’-promoter region of Cdx2 VDR polymorphism and serum vitamin D association in female genital tuberculosis.

Document Type : Original Article


1 Department of Obstetrics & Gynecology, King George's Medical University, Lucknow,U.P

2 Department of Obstetrics & Gynecology, King George’s Medical University, Lucknow (226003), Uttar Pradesh, India

3 Department of Obstetrics & Gynecology, All India Institute of Medical Sciences, Raebareli (229405), Uttar Pradesh, India

4 Dept. of Microbiology, King George’s Medical University, Lucknow (226003), Uttar Pradesh, India


Background: Globally, genital tuberculosis (GTB) is a substantial cause of female infertility. The vitamin D receptor (VDR), a member of the nuclear receptor superfamily, mediates the immunological function of vitamin D3, activates macrophages, and vitamin D deficiency has been linked to FGTB risk.

Objective: The aim of this study is to assess the relationship between risk of FGTB and the vitamin D receptor gene polymorphism (Cdx2) and serum vitamin D level.

Methods: 150 confirmed FGTB cases and 150 healthy controls were recruited. Serum vitamin D level was measured by ELISA. Genomic DNA was extracted and the genotyping of VDR-Cdx2 polymorphism was performed by T-ARMS-PCR.

Results: Serum vitamin D levels were significantly lower among FGTB patients. The frequency of A allele was 68% in FGTB and 48.6% in control; A allele was significantly associated with increased risk of FGTB [OR = 2.24; 95 % confidence interval [CI]; p< 0.0001] however, the frequency of G allele (32% in FGTB and 51.3% in control; G allele did not show significant risk of FGTB [OR = 1.10; 95 % confidence interval [CI]; p = 0.67]. A significant association was found between VDR Cdx-2 AA (p <0.001) genotype and vitamin D level.

Conclusion: Genotype frequencies of VDR gene polymorphism and Vit D were found significantly association leads, VDR dysfunction, could increase FGTB risk. Understanding the synergism between VDR polymorphism and vit D in FGTB will be important to identifying the new prognostic tool and target for therapy in vit D deficient individuals.


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