Plasmid profiling analysis of some meropenem-resistant Enterobacteriaceae isolates from poultry farms in Ado Ekiti, Nigeria

Ekundayo, Olajumoke Kemi; Oyinloye, Josiah Mofoluwaso Adedeji

doi:10.21608/mid.2024.220023.1558

Plasmid profiling analysis of some meropenem-resistant Enterobacteriaceae isolates from poultry farms in Ado Ekiti, Nigeria

Document Type : Original Article

Authors

¹ Biological Science Department, Bamidele Olumilua University of Education, Science, and Technology Ikere Ekiti, Ekiti State, Nigeria

² Biological Science Department, Afe Babalola University Ado Ekiti, Nigeria

10.21608/mid.2024.220023.1558

Abstract

Background: The presence of antibiotic-resistant microorganisms in food animals especially poultry is of great concern globally. Aim: This research was carried out to detect and characterize plasmid carriage and profiles among members of Enterobacteriaceae from different poultry breeds especially broilers, layers, and noilers chicken in Ado Ekiti, Ekiti State, Nigeria. Methods: A total of 93 rectal samples were collected and isolation and identification were done using standard microbiological techniques. The bacterial isolates were identified using DNA sequencing analysis. Antibiotic susceptibility testing was carried out using oxoid single disc polymyxin b, gentamycin, fosfomycin, nitrofurantoin, meropenem, and tigecycline. Plasmid profile and plasmid curing were also done to determine molecular weight and route of resistance whether plasmid or not. Results: A total of 7 bacterial isolates which include Br1 (Pseudomonas monteilii) and Br7 (Escherichia coli) from broilers. Cl29 (Shigella flexneri) CL33 (Proteus mirabilis), and CL33w (Proteus mirabilis) were isolated from layers, and Cn 3 and Cn14 (Bacillus cereus) were isolated from noiler in Ado Ekiti and they were resistant to meropenem. From the plasmid profiling analysis, it was revealed that all the isolates harbored plasmids. The plasmid sizes ranged from approximately 8000-10000bp and resistance was mainly plasmid-mediated. Conclusion: The findings of this study prove the consequences of antibiotic resistance and its usage among poultries are alarming as well as the relationship of plasmid with antibiotic resistance which necessitates proper surveillance of antibiotic usage in developing countries, especially among poultry farms.

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