Molecular detection of colistin resistant gene (MCR-1) of Gram-negative bacilli from clinical isolation in Khartoum State among child under 15 years

Abdalla Hamed, Mustafa Adam; Hammad, Mohammed Aldai; Idris, Alkhair Abd Almahmoud

doi:10.21608/mid.2024.252068.1696

Molecular detection of colistin resistant gene (MCR-1) of Gram-negative bacilli from clinical isolation in Khartoum State among child under 15 years

Document Type : Original Article

Authors

¹ Department of Graduate College and Scientific Studies, Gharb El-Niel College-Sudan.

² Department of Medical Laboratory Sciences- Dar ALaloum College-Sudan

³ Department of human biology and histology. Ahfad University for Women, Sudan

10.21608/mid.2024.252068.1696

Abstract

Background: Colistin resistant gene is uncommon, but recently several species of Gram negative bacteria have been MCR-1 such as A.baumannii, K.pneumoniae and P.aeruginosa. Many studies showed that the transfer of resistance genes is more effective than chromosomal mutation, the colistin resistant genes are located in a DNA sequence in plasmid which can be transferred from one plasmid to another. Material and Methods: In total, 100 consecutive non-repetitive Gram-negative bacilli isolates were obtained from different clinical specimens of patients less than 15 years. The isolates were reidentified by standard biochemical tests. The isolates were subjected to antimicrobial susceptibility testing. PCR was also used to detect the presence of mcr1 gene in the isolates. Results: Totally, 50 (50%) isolates were resistant to colistin for all isolates, PCR technique was done for detection of mcr1 gene, only (7) resistant samples carried mcr1 gene. Conclusions: Number of Escherichia coli against colistin and carried mcr1 gene was 2 (20%). Klebsiella spp carried mcr1 gene was 2(8%). Pseudomonas spp against colistin and carried mcr1 2(15%). Finally, the number of Proteus spp against colistin and carried mcr1 was 1 (25%)..

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