Antimicrobial activity of carbol fuchsin dye: Is there efficacy against Candida albicans?

Document Type : Correspondence


1 Department of Microbiology, Al-Shomali General Hospital, Babylon Health Directorate, Babylon, Iraq

2 Department of Medical Laboratory Technology, College of Medical Technology, The Islamic University, Najaf, Iraq


Candida albicans (C. albicans) developed resistance to multiple antibiotics recently, making them difficult to treat with standard therapies. So using alternative strategies such as dyes to treat C. albicans is urgent. Strong carbol fuchsin (SCF) is a red-colored dye commonly used in histology to stain biological tissues and microorganisms. SCF has a high affinity for acidic structures such as nucleic acids and polysaccharides, making it useful for staining cell nuclei and cartilage. In the current study, C. albicans isolated from 30 positive culture patients from three sites (vagina, oral, and urine) and cultured in sabouraud dextrose agar and confirmed by germ tube test. The well diffusion method used to evaluate the activity of SCF and diluent carbol fuchsin (DCF) stains as well as disc diffusion method after overnight incubation for antibiotic sensitivity test of fluconazole 25 µg as control agents; the diameter of the inhibited growth is measured in millimeter. 


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