Biosurfactants production and antimicrobial effects in environmental isolates of Pseudomonas aeruginosa

Document Type : Original Article


1 Department of Microbiology, Gorgan Branch, Islamic Azad University, Gorgan, Iran

2 Department of Biology, Gorgan Branch, Islamic Azad University, Gorgan, Iran


Background: Surfactants are a group of polar molecules with two parts, hydrophilic and hydrophobic that usually stay between two phases with different polarity. This property enables the reduction of surface and interfacial tension of liquids and leads to the formation of microemulsions. Methods: In this study, the production of rhamnolipid from Pseudomonas aeruginosa was investigated using different amounts of carbon including crude oil, engine oil, burnt oil, and coconut oil. Result: In the screening results of 29 isolates, 15 isolates in crude oil, 8 isolates in engine oil, 7 isolates in brake fluid, 7 isolates in burnt oil, and 7 isolates in coconut oil showed positive results. The best result was obtained using crude oil and engine oil, brake fluid, burnt oil, and coconut oil all producing the same amount of rhamnolipid from Pseudomonas aeruginosa. Conclusion: Pseudomonas aeruginosa uses the majority of oils, especially crude oil, to produce rhamnolipid, and this feature significantly increases the decomposition of hydrocarbons.


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