Quantification of adenovirus-F in the river Owena and the water’s physicochemical properties

Document Type : Original Article


1 Department of Microbiology, Federal University of Technology, Akure, Nigeria.

2 Inqaba Biotech West Africa, Moniya 200136, Ibadan, Nigeria


Background:  Water quality monitoring is useful for promoting sustainable development of water resources. Objective: The aim of this study was to determine adenovirus concentrations in water samples from River Owena, Nigeria. Methods: The physicochemical characteristics of the water samples were determined immediately after collection using standard methods. A quantitative SYBR green-based real-time PCR assay for detecting human adenovirus-F (HAdV-F) was optimized using DNA extracted with a ZymoBIOMICS™ DNA Miniprep kit from filtered water samples. Results: Findings revealed that HAdV-F genes with end relative fluorescence units (RFUs) ranged from 7206.31 to 14248.55 and corresponding quantification cycles (Cq) ranged from 13.32 to 18.38, while the non-template control (NTC) was 31.71 in a 44-cycle reaction. Water temperature ranged from 34 to 36℃ and pH 6.4 to 7.4, with total solids between 945 and 994 mg/l. Conclusion: Adequate treatment of the water from the river must be a priority by residents who rely on the water for domestic activities.


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