Antibiogram and antimicrobial potency of Chromolaena Odorata leaf crude extracts on post-operative wound bacterial flora from hospital settings

Document Type : Original Article


Department of Microbiology, Federal University of Technology, Akure, Nigeria


Background: This study is aimed at identifying the bioactive components in Chromolaena odorata active against multiple antibiotics resistant bacteria (MARB) isolated from post-operative wound infections. Methods: A total of 69 septic wound samples were collected from patients (male and female) with clinically suspected post-operative wound infections. All methods were conducted using standard microbiological techniques. Results: Staphylococcus epidermidis was susceptible to gentamycin (10µg) at 24.50±0.50 mm, streptomycin (30µg) at 20.00±0.00 mm, ciprofloxacin (10µg) at 28.00±0.00 mm and amoxicillin (30µg) and are resistant to all other antibiotics tested. The 300 mg/ml concentration of the methanol crude extract had the highest zone of inhibition (ZOI) at 22.55±0.55 mm on Staphylococcus aureus and 22.30±0.30 mm ZOI was recorded on Enterococcus faecalis while the least ZOI at 6.45±0.25 mm on Streptococcus pyogenes. 12.5 mg/ml was the least inhibitory concentration on Klebsiella pneumoniae while 50 mg/ml was recorded as the highest minimum bactericidal concentration (MBC) on Staphylococcus epidermidis and Pseudomonas aeruginosa at 50 mg/ml. Conclusion: The identification and characterization of bioactive compounds from Chromolaena odorata leaves could help develop more effective antimicrobial treatments for the management of post-operative wound infections. Further research is needed to isolate and characterize the active bioactive chemical compounds in Chromolaena odorata responsible for the antimicrobial activity and to evaluate their safety and efficacy in clinical settings for the treatment of post-operative wound infections.


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