Antimicrobial resistance in Escherichia coli and Salmonella spp. isolated from table egg shells and its contents sold at different markets in Peshawar, Pakistan

Document Type : Original Article


1 Pure Health Laboratory, Mafraq Hospital, Abu Dhabi, UAE

2 Department of Microbiology, Sarhad University of Science and Information Technology, Peshawar, Pakistan.

3 Pure Health Laboratory, Mafraq Hospital, Abu Dhabi, United Arab Emirates. Ascencia Business School, College de Paris, France.

4 Pure Health Laboratory, Mafraq Hospital, Abu Dhabi, United Arab Emirates. School of Life Science, Manipal Academy of Higher Education, Dubai, United Arab Emirates.

5 Federal General Hospital, Islamabad, Pakistan.

6 Bacha Khan University, Charsadda, KP, Pakistan.

7 CECOS University of Information Technology and Emerging Sciences, Peshawar, Pakistan.

8 Pure Health Laboratory, Mafraq Hospital, Abu Dhabi, United Arab Emirates.

9 Department of Microbiology, Harbin Medical University, China.


Background: The current study was carried out with the aim to reveal the presence of Escherichia coli (E. coli) and Salmonella species (spp.) in table eggs sold for human use in Peshawar, and their antimicrobial resistance to different drugs used in Pakistan for poultry and human practices. Methods:  A total of 80 eggs were bought from different shops. E. coli and Salmonella spp < /em>. were isolated from 85.36% (70) and 14.63% (12) of the total collected egg samples (80), respectively. The antibiotics susceptibility test was done using the disk diffusion method. Results: The shells of eggs showed significantly higher contaminations as compared to egg internal contents for both E. coli and Salmonella spp < /em>. The isolated organism presented a significant resistance to multiple drugs. They were found to be completely resistant to penicillin G, nitrofurantoin, oxacillin, and sulfamethoxazole/trimethoprim. Furthermore, Salmonella spp. was also completely resistant to tetracycline. The susceptibility pattern of both these isolates was quite different for some antimicrobials, as Salmonella isolates were completely sensitive to neomycin, whereas 100% susceptibility was observed in E. coli isolates to amikacin, amoxicillin-clavulanic acid, levofloxacin, and neomycin. In addition, resistance frequency among isolates of ­E. coli against amoxicillin (77.14%), minocycline (54/28%), streptomycin (80%), and tetracycline (80%), and in Salmonella spp. against amikacin (50%), amoxicillin (66.66%), amoxicillin-clavulanic (66.66%), levofloxacin (33.33%), minocycline (50%) and streptomycin (83.33) were also high. Conclusion: Hence it can be concluded from the present study that the table eggs sold in the markets of Peshawar Pakistan are infected with multidrug resistance (MDR) strains of E. coli and Salmonella spp < /em>.


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