Microbiological profile of blood stream infection: Genetic relatedness of carbapenemase producing klebsiella Pneumoniae isolates using ERIC PCR

Document Type : Original Article

Authors

1 Department of Medical Microbiology and Immunology, Faculty of Medicine, Tanta University, Egypt.

2 Department of Clinical Pathology, Faculty of Medicine, Tanta University, Egypt.

3 Department of Hygiene and Preventive Medicine (Zoonoses), Faculty of Veterinary Medicine, Kafr Elsheikh University, Egypt.

4 Department of Anesthesiology, Surgical Intensive Care and Pain Medicine, Faculty of Medicine, Tanta University, Egypt.

Abstract

Background: There has been an expansion in antibiotic resistance among Enterobacteriaceae over the previous ten years raising serious questions about global health. Our objectives were to isolate the carbapenem-resistant Gram-negative bacilli that cause blood stream infections and investigate the genetic relatedness between carbapenemase producingKlebsiella pneumoniae (K. pneumoniae) isolates. Methods: A total of 255 blood specimens were withdrawn into blood culture bottles and incubated, pathogenic microorganisms were identified, antibiotic susceptibility was done for Gram-negative isolates. Carbapenemase producers were screened for molecular detection of carbapenemase and extended spectrum β-lactamase genes. Results: One hundred and thirty-three (133) pathogens were isolated, of these 77 Gram-negative isolates (including 32 K. pneumoniae,18 E. coli, 12 Acinetobacter, 9 Pseudomonas and 6 Enterobacter cloacae). Out of them, 55 were carbapenem resistant by phenotypic method. 23/55 of them were harboring carbapenemase genes by PCR ERIC, results showed that Anesthesia intensive care unit (ICU) isolates showed >90% genetic similarity while isolates from Chest and Internal medicine ICUs showed <70% similarity. Conclusion: Increased awareness, persistent observation, and strict adherence to the recommendations for antibiotic stewardship should be used to limit spread of carbapenem resistant Gram-negative bacilli.

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