Document Type : Original Article
Microbiology and Immunology Department, Faculty of Medicine, Benha University, Egypt
Department of Internal Medicine, Al Quwayiyah General Hospital, Saudi Arabia
Background:Pseudomonas aeruginosa is one of the common emerging multidrug-resistant causative bacteria which causes healthcare associated infections that leads to increased morbidity and mortality rates. Aim: Detection of resistance pattern of studied isolates to different antipseudomonal drugs and to determine the prevalence of carbapenem resistant Pseudomonas aeruginosa and to detect the involved carpabenemasesgenes among resistant isolates. Methods:The study was done from November 2021 to April, 2022 in Al-Quwayiyah general hospital. Eighty six Pseudomonas aeruginosa isolates were collected. Identification of isolates and antimicrobial susceptibility testing were done using vitek-II machine. Carbapenem resistance was detected by modified Hodge Test then confirmed using multiplex PCR for the detection of blaVIM, blaKPC, blaIMP, blaNDM-1, blaOXA-48, blaGIM, blaSPM and blaSIM genes. Results: Thirty two (37.20%) strains were carbapenem-resistant Pseudomonas aeruginosa(CRPA) as detected by multiplex PCR. Among these 32 strains the resistance was 100% to imipenem, meropenem and ciprofloxacin. The isolates had least resistance to aztreonam, 21.88% and colistin, 31.25%. Among 32 CRPA isolates 23 (71.88. %) were multi-drug resistant, 19 (59.38%) were Extensively-drug resistant. PCR identified the presence of blaOXA-48 in 15 (46.88%) isolates, blaVIM gene in 10 (31.25%) isolates and blaNDM in 12 (37. 5%) isolates. On the other hand, blaGIM, blaIMP and blaSIM were only detected in 2 isolates for each and blaKPC detected in one isolate only. Conclusion: The prevalence of CRPA was high (37.2%). The appropriate study of the antimicrobial resistance molecular mechanisms will help in management of CRPA patients and implementation of infection control procedures.