Ghandour, A., Mohamad, W., Bakry, R., Ahmed, A., Elsherbiny, N. (2022). Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli. Microbes and Infectious Diseases, 3(2), 366-377. doi: 10.21608/mid.2022.118857.1242
Aliaa Mahmoud Ghandour; Wegdan Abdelhamid Mohamad; Rania Mohamed Bakry; Asmaa Omar Ahmed; Nahla Mohamed Elsherbiny. "Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli". Microbes and Infectious Diseases, 3, 2, 2022, 366-377. doi: 10.21608/mid.2022.118857.1242
Ghandour, A., Mohamad, W., Bakry, R., Ahmed, A., Elsherbiny, N. (2022). 'Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli', Microbes and Infectious Diseases, 3(2), pp. 366-377. doi: 10.21608/mid.2022.118857.1242
Ghandour, A., Mohamad, W., Bakry, R., Ahmed, A., Elsherbiny, N. Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli. Microbes and Infectious Diseases, 2022; 3(2): 366-377. doi: 10.21608/mid.2022.118857.1242
Performance of chromID® CARBA-SMART medium and carbapenemase inhibition method for the detection of carbapenemases among Gram negative bacilli
1Medical Microbiology and Immunology Department, Faculty of Medicine, Assiut University, Assiut, Egypt
2Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut University, Assiut-Egypt.
3Department of Oncological Clinical Pathology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt.
4Department of Clinical Pathology, Faculty of Medicine, Assiut University, Assiut, Egypt
Abstract
Background:Early detection of carbapenemase enzymes among Gram negative bacilli (GNB) is mandatory to prevent their spread. Objective: The goal of this study was to evaluate the performance of chromID® CARBA-SMART medium and carbapenemase inhibition method (CIM) for detection of carbapenemases in GNB. Methodology: A total of 142 GNB isolates were collected and tested using Vitek-2® system for identification and antimicrobial susceptibility testing The carbapenem resistant (CR) GNB were tested for carbapenemase production by phenotypic methods; chromID® CARBA-SMART medium and CIM. Carbapenemase genes (𝑏𝑙𝑎NDM-1, blaSIM-1,𝑏𝑙𝑎VIM-2, 𝑏𝑙𝑎KPC-1, blaGIM-1,blaSPM-1 and 𝑏𝑙𝑎OXA-48) were detected by PCR. Results: By minimal inhibitory concentration (MIC), 111 (78.17 %) of the 142 isolates were shown to be carbapenem resistant. Sensitivity and specificity of CIM and ChromID® CARBA-SMART medium were (100% and 66.7% respectively) for CIM and (86.7% and 100% respectively) for the medium. Resistance to carbapenem was associated with high percentages of resistance to many antibiotic classes. Carbapenemase genes were detected in 82% (91/111) of CR GNB with 𝑏𝑙𝑎NDM-1 (58.6%) and 𝑏𝑙𝑎OXA-48 (55.9 %) having the highest prevalence, followed by 𝑏𝑙𝑎KPC-1 (36 %) then 𝑏𝑙𝑎VIM-2 (10.8%) and lastly blaSPM-1 (3.6 %) and blaSIM-1 (1.8 %). The blaGIM-1 gene was not detected in any isolate. Conclusion: Carbapenemase inhibition method was found to be a very sensitive, easy, and cheap test for carbapenemase detection but needs the addition of ChromID® CARBA-SMART medium to improve the specificity of the test. This study has a high prevalence of carbapenemases among isolates with potential of rapid spread necessitating need for phenotypic tests.
Top