Elshahat, M., Aboelized, E., Abd El Rahman, R., Nabil, M., Yassin, A. (2021). Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance. Microbes and Infectious Diseases, 2(2), 343-351. doi: 10.21608/mid.2021.64414.1127
Mohamed Elshahat; Esam Aboelized; Raafat Abd El Rahman; Mohamed Nabil; Ayman Yassin. "Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance". Microbes and Infectious Diseases, 2, 2, 2021, 343-351. doi: 10.21608/mid.2021.64414.1127
Elshahat, M., Aboelized, E., Abd El Rahman, R., Nabil, M., Yassin, A. (2021). 'Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance', Microbes and Infectious Diseases, 2(2), pp. 343-351. doi: 10.21608/mid.2021.64414.1127
Elshahat, M., Aboelized, E., Abd El Rahman, R., Nabil, M., Yassin, A. Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance. Microbes and Infectious Diseases, 2021; 2(2): 343-351. doi: 10.21608/mid.2021.64414.1127
Association between mutations in GYR A/B and PAR C/E genes of Acinetobacter Baumannii clinical isolates and Fluoroquinolones resistance
1Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Cairo, Egypt
2Department of Microbiology and Immunology, Military Medical Academy, Cairo, Egypt
3Department of Microbiology and Immunology, Faculty of Medicine, Al-Azhar University, Assuit, Egypt
Abstract
Background: Acinetobacter baumannii (A. baumannii) is an important organism in hospital acquired infections. A. baumannii has emerged as Quinolones resistant organism.The maximum crucial mechanism of fluoroquinolones resistance is the mutation in genes that encode the subunits of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). Objectives: determination of mutations of gyrA/B and parC/E genes using Restriction Fragment Length Polymorphism PCR (RFLP - PCR) among fluoroquinolones resistant clinical isolates of A. baumannii showed in ICU patients at Al-Azhar University hospitals. Subjects & Methods: The study was conducted on 100 isolates of A.baumannii which subjected to molecular study of mutations of gyrA, gyrB, parC and parE genes beside determination of minimal inhibitory concentration (MIC). Results: The most A. baumannii isolates had MIC >128 µg/ml (61.1%). The resistant isolates showed combind mutations in both gyrA& parC genes most frequently (79.2%) then gyrA (9.7%), parC (8.3%) and gyrB (2.8%). Conclusion: Mutations in gyrA and parC are the most common in fluoroquinolones resistant A. baumannii isolated from Al-Azhar University hospitals
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