Predominance of blaKPC carbapenemase gene in multi-drug resistant Pseudomonas aeruginosa isolated from ICUs and surgical wards in Egypt

Hassuna, Noha A.; Koriem, Nehal E.; Zaki, Shaimaa Hassouna; Abd El Fattah, Medhat; Abdelmoaty, Amani Mohammed; Ibrahem, Reham A.

doi:10.21608/mid.2024.279851.1883

Predominance of blaKPC carbapenemase gene in multi-drug resistant Pseudomonas aeruginosa isolated from ICUs and surgical wards in Egypt

Document Type : Original Article

Authors

¹ Medical Microbiology and Immunology Dept., Faculty of Medicine, Minia University, Minia, Egypt

² Botany and Microbiology, Department, Faculty of Science, Beni-Suef University, Beni Suef, Egypt

³ Pharmaceutics, Faculty of Pharmacy, Beni-Suef University, Beni-Suef University, Beni Suef, Egypt

⁴ Microbiology and Immunology Department, Faculty of Pharmacy, Minia University, Minia, Egypt.

10.21608/mid.2024.279851.1883

Abstract

Background: The last-resort classes of antibiotics with the best success in treating severe Pseudomonas aeruginosa infections caused by drug-resistant P. aeruginosa are carbapenems. So, we aimed to assess the frequency of carbapenem-resistant genes in P. aeruginosa. Methods The samples isolated from critically ill patients at Intensive Care Units (ICUs) and Surgical Wards in Minia, Egypt were examined to produce imipenem-resistant genes phenotypically by Modified Hodge test (MHT) and Modified carbapenem inactivation method (mCIM) test and genotypically by PCR. Results: Fifty isolates (64%) of P. aeruginosa were resistant to carbapenems (imipenem and meropenem). Of which: 16 (32%) were (MHT) positive and 26 (52 %) were (mCIM)-positive. Regarding resistance genes, bla_IMP, bla_VIM, and bla_KPC were detected in 14%, 20%, and 48% of the carbapenem resistant Pseudomonas aeruginosa (CRPA) isolates; respectively, while bla_GIM, bla_SIM, bla_NDM-1and bla_SPM-1 were not detected. Conclusion: There is a high prevalence of multi-drug resistant MDR strains of P. aeruginosa, Doctors should pay attention to carbapenem resistance. CRPA isolates were detected phenotypically with mCIM test which is much more sensitive than MHT and genotypically with bla_KPCgene being the most frequently detected gene

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