Potential probiotics from human breast milk with promising cholesterol-reduction and anti-tumour effects

Abd El Mawla, Amany; Mehanna, Nayra Shaker; Sallam, Marwa Kamal; Kamel, Zeinat; Mohamed, Mahmoud S.M.

doi:10.21608/mid.2024.271239.1811

Potential probiotics from human breast milk with promising cholesterol-reduction and anti-tumour effects

Document Type : Original Article

Authors

¹ Department of Botany and Microbiology, Faculty of Science, Cairo University, 12613, Giza, Egypt.

² Professor of Dairy Microbiology -Head of Dairy Science and Probiotic Department - NRC- Dokki, 12311, Giza, Egypt

³ Department of Medical Microbiology and Immunology, Faculty of medicine, Cairo University

⁴ Department of Botany and Microbiology, Faculty of Science, Cairo University, 12613, Giza, Egypt

10.21608/mid.2024.271239.1811

Abstract

Objectives: To isolate probiotic bacteria from human breast milk and test their health benefits as cholesterol reduction and human DNA protection. Methods: Potential probiotic bacteria were isolated from human milk. The probiotic properties were tested namely their ability to survive acidic pH, tolerate bile salts and antimicrobial activities. Serum cholesterol reduction ability and cytoprotective effect on Hep-2 cells were studied. Results: A total of 48 different isolates were purified from 12 breast milk samples. They all tolerated bile salts while 81% were proved to be resistant to acidic pH and had antimicrobial effects against different pathogens . Three isolates showed high ability to reduce cholesterol from 19.4-22.6%. This percentage of cholesterol reduction was improved in presence of bile salt to be 48.7-54.7%. Four isolates were proved for a protective effect against the H₂O₂ cytotoxicity by decreasing tail moment up to 2.8 and 15.6% DNA concentration in the tail of the examined cells. Phenotypic characteristics and 16S rDNA sequence analyses were used to identify the promising isolates as Pediococcus pentosaceus, Enterococcus durans, and Enterococcus faecium strains. Conclusion: Probiotic strains isolated from human milk in the study had a dual beneficial role; human health benefit as well as cytoprotective activities.

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