Antitumor impact of Clostridium sporogenes endospores against diethyl nitrose amine induce hepatocellular carcinoma in rats

Document Type : Original Article

Authors

1 Botany and Microbiology department, Faculty of Science, AlAzhar University, Cairo, 11884, Egypt

2 Radiation biology department, Egyptian atomic energy authority, Cairo, Egypt

Abstract

Background: The exploration of bacterial endospores as potential antitumor agents has garnered increased attention in recent years. This study investigates the impact of gamma radiation-attenuated Clostridium sporogenes endospores as modifiers of immune regulation on tumor growth, with the aim of refining therapeutic protocols for hepatocellular carcinoma. Methods: Hepatocellular carcinoma was induced in adult male albino rats using diethylnitrosamine (DEN) (20mg/kg b.wt/day) orally injected five times a week for 6 consecutive weeks) and subsequently treated with endospores (intravenous injection of endospores  once at a dose of (60 million of spores /rat) and samples were taken from rat after one month of endospore injection). Multiple parameters, including interferon gamma (IFN-γ), nuclear factor-κB (NF-κB), tumour necrosis factor alpha (TNF-α), antioxidant enzymes, malondialdehyde (MDA), and liver function enzymes were assessed. Results: Results demonstrated a remarkable suppression of tumor proliferation coupled with the induction of enduring immunity through IFN-γ stimulation. The levels of inflammatory markers associated with tumor promotion (NF-κB and TNF-α) witnessed significant improvement. This was paralleled by enhancements in antioxidant status and a reduction in lipid peroxidation in liver tissues of the endospore-treated rats compared to the untreated rats. Histopathological examinations, along with elevated liver function enzymes, provided additional support for these observed improvements. Conclusion: This study offers compelling evidence for the potential of endospores in modulating immune responses and targeting tumor cells, suggesting their plausible roles as adjuvants to augment cancer therapeutic protocols

Keywords

Main Subjects

Microbes and Infectious Diseases

Articles in Press, Accepted Manuscript
Available Online from 07 February 2024

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